A spectrometer is a general term for an optical instrument. Spectrometric methods convey a large group of analytical methods based on molecular or atomic spectroscopy. Spectroscopic instruments will typically contain a stable source of radiant energy, a transparent veil for the sample or blank, a component to separate different wavelengths of light, a detector, and a readout/processor.
Photometers are simpler in design compared to spectrophotometers. They are less expensive, more convenient, easier to maintain, and good for field work. Some photometers include: visible photometers, probe-type photometers, and general purpose photometers using adjustable filters.
Spectrophotometers may have a few more mirrors, slits, a grating, and maybe a filter to top if off. Some examples include the popular Spectronic 20, Varian Cary 100, or even miniature fiber-optic spectrometers.
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A flame photometer measures the intensity of light emitted by excited atoms in a flame to determine the concentration of specific elements in a sample. An atomic absorption spectrometer measures the absorption of light by neutral atoms in a sample to quantify the concentration of specific elements. The main difference is in the method of detection: emission for flame photometry and absorption for atomic absorption spectrometry.
A photometer simply measure the total lumens of a light source, while a spectrophotometer will measure a whole spectral of that same light source. Hence a spectrophotometer will give us more details about how the light is acting on the spectral band.
In atomic absorption spectroscopy we deal with atoms at ground stage, these atoms absorb a characteristic wavelength of radiations coming from the source which is commonly a hollow cat-hod lamp. It requires high energy and the resonance line is below 300nm.
flame emission spectroscopy deals with atoms at excited state, atoms are first excited by absorbing characteristic light from the source which must be a flame, then atoms fall back to ground state losing same wavelength. low energy is favourable and resonance line is above 300nm.
The UV/Vis passes light through the sample, measuring the light the sample absorbs; the AA aspirates the sample by exciting the molecules at a specific wavelength that allows you to measure only the ion you want! Basically the UV/Vis measures the sample as a whole and passes light through it; your sample is still available. The AA uses your sample to make the measurement, but is more selective to what you want to be measured within the sample ( can be used with contaminants present).
flame photometry is a type of atomic EMISSION spectroscopy. The sample is excited (raised to a high temperature), causing the emission of light. the wavelength of the emitted light is a function of the energy of the excited electrons, so each element has a characteristic set of wavelengths. usually a single wavelength is detected and the intensity of the emission is used to calculate concentration.
Atomic adsorption works in the reverse way. A light of a standard wavelength (a wavelength characteristic of the target element) is passed through a flame containing the unknown substance, and the concentration of the target element is determined by the reduction in the energy of this light as it passes through the flame. the light is adsorbed by the electrons in the target element, kicking them into a higher orbit or completely out of the atom, depending on the energy involved.
basically, one method involves the emission of the energy as an excited electron kicks back down to a lower state, and the other involves the adsorption of energy as an electron is kicked up an energy state. Same basic principle-change in electron energy relates to light of a specified wavelength and the change in the amount of that light can be measured and converted to a concentration.
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Spectroscopy is a field of study, a spectrophotometer is an instrument you might use in that field.
You've effectively asked "what is the difference between carpentry and a hammer?"
A colorimeter is a scientific instrument that measures the intensity of light passing through a pure sample. In biology, colorimeters are used to monitor the growth of cultures. As the culture grows, the growing medium becomes more cloudy and absorbs more light.
A spectrophotometer consists of two instruments, namely a spectrometer for producing light of any selected color (wavelength), and a photometer for measuring the intensity of light. For solutions where intensity of light is proportional to the concentration, we use spectrophotometer.
Yes - the amount of absorption can depend on the frequency.
Algae lack specialized tissues like roots, stems, and leaves that are present in plants. Instead, algae have simple structures for attachment and nutrient absorption.
The resonance line in atomic absorption spectroscopy refers to the specific wavelength of light that matches the energy difference between the ground state and an excited state of an atom. When the atom absorbs light at this resonance wavelength, it undergoes a transition to an excited state, allowing for the identification and quantification of the element present in the sample.