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Tailing peaks are a known problem in chromatography (both HPLC and GC). It means the analytes are experiencing an out-drawn (extended) retention time and is usually due to one of these factors:
- Contaminated or active injector liner, seal or column
- Dead volume due to poorly installed liner or column
- Ragged column end
- A bad match between the polarities of the stationary phase and the solvent
- A cold region in the sample flow path (GC)
- Debris in the liner or column
- Injection takes too long
- Split ratio is too low (GC)
- Overloading the inlet
- Some types of compounds such as alcoholic amines, primary and secondary amines and carboxylic acids tend to tail
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Professor
Beau
BlakeTailing in chromatography refers to the phenomenon where the peak in the chromatogram is elongated on one side, leading to a distorted peak shape. This can be caused by factors such as non-ideal interactions between the analyte and the stationary phase, resulting in slower elution. Tailing can affect the accuracy and precision of peak identification and quantification in chromatographic analysis.
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What are the causes of tailing in chromatography?
Tailing in chromatography can be caused by factors such as non-specific interactions between the analyte and the stationary phase, column overload, sample matrix effects, or inaccuracies in the injection process. These factors can lead to peak distortion, decreased resolution, and decreased sensitivity in chromatographic separations.
What is meant by tailing a spot in Chromatography?
Tailing in chromatography refers to a peak shape that has a gradual slope on the downside of the peak, resembling a tail. This can be caused by interactions between the analyte and the stationary phase, leading to slower elution. Tailing peaks can affect resolution and accuracy of the analysis.
What are the different types of chromatography?
The main types of chromatography include gas chromatography (GC), liquid chromatography (LC), thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). Each type of chromatography separates compounds based on their differing affinities for a mobile phase and a stationary phase.
How many types of Chromatography are there?
There are four main types of chromatography: gas chromatography (GC), liquid chromatography (LC), thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). Each type of chromatography has specific applications and uses in separating and analyzing chemical compounds.
Why ethanol not suitable in Gas chromatography?
Its alcohol so it can purify the chemicals used in the experiment. Furthermore, it will travel up the filter paper better than water will. Ethanol is pure whereas water may have other things in it such as chlorine is in tap water
