The molar extinction coefficient (also sometimes called molar absorbtivity coefficient) is a measure of how strongly a solution of a substance absorbs light (the value depends on the particular wavelength of light used). By passing light through a solution and determining how much of the light is absorbed, you can use the path length and molar extinction coefficient to determine the concentration of the solution.Look up "Beer-Lambert law" if you want details.
We would need to know the path length and the molar extinction coefficient to answer that question. If you know these, it's an extremely simple matter of Beer's Law and algebra.
One can purchase BSA optics from various websites like Amazon or eBay. One can also buy BSA optics directly from the official BSA optics website itself.
To make a 1 mg/mL solution of BSA (bovine serum albumin), weigh out 1 gram of BSA and dissolve it in a total volume of 1 liter of water or buffer solution. Make sure to mix well until the BSA is fully dissolved. This will give you a 1 mg/mL solution of BSA.
To prepare a 10ml solution containing 1 mole of BSA (Molecular weight 66000 g/mol), you would need 66g of BSA. To prepare a 100ml solution containing 1 mole of BSA, you would need 660g of BSA. Remember to adjust the volume accordingly after dissolving the BSA to ensure accurate concentration.
Molar extinction coefficient of phenol ret at 610nM is 22 mM-1 cm-1
Molar extinction coefficient is depend on intensity of the colour of solution.If the solution has high intensity of colour, molar extinction coefficient is high.So when considering CoCl2 and KMnO4, CoCl2 has low colour intensity. KMnO4 solution has much intense purple colour.Therefore its Molar extinction coefficient is high. By-Tharindu Chathuranga Ariyathilaka/Sri Lanka
In the beginning, no you need not cull the special coefficient
The molar extinction coefficient (also sometimes called molar absorbtivity coefficient) is a measure of how strongly a solution of a substance absorbs light (the value depends on the particular wavelength of light used). By passing light through a solution and determining how much of the light is absorbed, you can use the path length and molar extinction coefficient to determine the concentration of the solution.Look up "Beer-Lambert law" if you want details.
The extinction coefficient can refer to a few different measures how strongly a distinct medium absorbs light at a particular wavelength. The two most commonly referred to are molar absorptivity (which measures absorption per molar concentration) and the mass attenuation coefficient (which measures absorption per mass density).
The molar extinction coefficient of ADP at a specific wavelength varies depending on the solvent and experimental conditions. It is typically around 8100 M-1cm-1 at 259 nm for ADP in water. Make sure to consult a reliable source or perform your own measurements for accurate values.
You might get an aproximate answer with the formula here. http://www.proteinscience.org/cgi/reprint/4/11/2411.pdf
I am not some one of this background and so please correct me if I am wrong. I think molar extinction coefficient will be very less and according to molecular structure of any molecule (bond vibration etc..,) only some wavelengths will have reasonable absorption and for glucose those wavelenghts are 1550-1850 nm; 6450-5400 cm(-1) (first overtone) 2000-2500 nm; 4000-5000 cm(-1) (combination). These are the spectral windows in which glucose has significant absorption.
The extinction coefficient of crystal violet is approximately 89,000 M^(-1)cm^(-1) at a wavelength of 590 nm. This value indicates the molar absorptivity of crystal violet at this specific wavelength, which is commonly used for measuring the concentration of crystal violet in solution using spectrophotometry.
We would need to know the path length and the molar extinction coefficient to answer that question. If you know these, it's an extremely simple matter of Beer's Law and algebra.
Given, molar mass, coefficient ratio, molar mass, answer
15.4 * 103 at 260 nm pH 7.5