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Bacteria possess extra chromosomal DNA,called plasmids. Often it carries functional genes for the resistance of bacteria (example: Aromotic compound degrading genes).
Plasmid curing is a process of completely removing plasmids of bacteria by means of chemical agents such as Acriflavin or acridine orange!
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Maxine
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EzraCuring of a plasmid refers to the loss of the plasmid from a bacterial cell. This can happen through the natural loss of the plasmid during cell division or through the use of specific treatments like chemicals or heat. Curing can be a useful tool in research to study the effects of plasmid presence or absence on bacterial traits.
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What is mode of action of acridine orange in plasmid curing?
Acridine orange can intercalate with DNA and induce frameshift mutations, leading to DNA damage and subsequent cell death. In plasmid curing, this can result in the loss of plasmids from bacterial cells, as the damaged DNA is eliminated during replication or cell division.
What is the term for a plasmid that contains a foreign gene?
Recombiant DNA
What is a helper plasmid?
A helper plasmid is a type of plasmid used in molecular biology to aid the replication and maintenance of another plasmid within a host cell. It often contains genes necessary for the replication or transfer of the target plasmid, and can provide other functions such as antibiotic resistance or visualization markers.
What occurs first in the production of a recombinant plasmid?
In the production of a recombinant plasmid, the DNA of interest (insert) and the plasmid vector are both cut with restriction enzymes to create compatible ends. These cut fragments are then ligated together using DNA ligase to produce the recombinant plasmid.
When is a plasmid considered a recombinant plasmid?
A plasmid is considered recombinant when it contains DNA sequences from two different sources that have been artificially combined, often through genetic engineering techniques like restriction enzyme digestion and ligation. This results in a plasmid with modified or additional genetic material compared to its original form.
