What is cell seeding density?

Answer 1

Cell seeding density refers the number of cells (usually per square centimeter) that are placed into a flask during cell passage. If growth is wanted a typical cell seeding density would be around 5,000 cells/square cm, however, if a high confluence is needed it may be more appropriate to seed cells around 50-60,000 cells/square cm.

How to determine cell seeding density-

look at the cells in a hemocytometer. This is a small plate that has very small squares etched into its surface, which allows for counting of individual cells underneath a microscope to determine the density of the cells in the cell suspension, and this the total number of cells can be approximated.

Answer 2

Cell seeding density refers to the number of cells that are plated onto a culture vessel, such as a dish or a well of a plate, at the beginning of a cell culture experiment. It is an important parameter that can affect cell behavior, growth, and experimental outcomes. It is typically expressed as the number of cells per unit area or volume.