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The main difference between ODS (octadecylsilane) and BDS (butylsilane) columns lies in the nature of the functional groups attached to the silica support. ODS columns have hydrophobic octadecyl groups which interact mainly through hydrophobic interactions, while BDS columns have more polar butyl groups that interact through a combination of hydrophobic and polar interactions. These differences can affect the selectivity and retention of compounds in column chromatography.
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