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Denaturants such as urea, SDS, guanidium hydrochloridecan denature proteins. Organic solvent such as alcohol can be also used to denature proteins. A combination of reducing agent DTTor beta-mercaptoethaol with heating at 90 degrees for 5 minutes about will completely kill the three dimensional structure of proetin and make it to its primary structure.

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Common chemicals that denature proteins include urea, guanidine hydrochloride, and detergents like sodium dodecyl sulfate (SDS). These chemicals disrupt the non-covalent interactions that maintain protein tertiary and quaternary structure, leading to protein unfolding and loss of function.

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9mo ago
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Q: What chemicals denature proteins?
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