Use regular beef broth which you have cooked down in a pan to half its original volume.
Alternatively, use a beef bouillon powder or paste (Bovril, Better-Than-Bouillon, etc.) diluted with half the recommended amount of water. You’ll need to reduce the amount of salt in the recipe too if you do this.
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Condensed broth typically needs to be diluted before use. Usually, condensed broth requires 1 part broth to 3 parts water for reconstitution. So, 10.75 ounces of condensed broth will yield about 43 ounces of reconstituted broth.
Beef broth provides nutrients and essential components such as amino acids, vitamins, and minerals that support the growth of microorganisms in a culture medium. It also helps maintain the pH balance and serves as a source of carbon and energy for microbial metabolism.
You can dip a variety of ingredients in a broth fondue, such as thinly sliced meats (beef, chicken, pork), seafood (shrimp, scallops), tofu, vegetables (mushrooms, bell peppers, zucchini), and noodles. Simply cook the ingredients in the broth until they are cooked to your liking.
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Peptone and beef extract are common nitrogen sources used in nutrient broth or agar. These ingredients provide essential amino acids and peptides that microorganisms require for growth.