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principle of microscopy includes 2 important aspects:

1. resolution and resolving power- the resolving power of a microscope is defined as the minimum distance by which 2 points must be separated in order for them to be perceived as 2 separate points rather than a fused image.

2. magnification- Linear (sometimes called lateral or transverse) magnification refers to the ratio of image length to object length measured in planes that are perpendicular to the optical axis. A negative value of linear magnification denotes an inverted image. Longitudinal

magnification denotes the factor by which an image increases in size, as measured along the optical axis.

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14y ago
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5mo ago

The two basic principles involved in using a microscope are magnification, which enlarges the image of the specimen, and resolution, which refers to the clarity and detail of the image produced by the microscope. These principles work together to allow for better visualization of small objects or structures that are not visible to the naked eye.

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10y ago

One principle of operating a microscope is that there is more than one lens that can be used. Another principle of operating a microscope is that items to be looked at should be in a saline solution to help see them more clearly.

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10y ago

In a simple microscope there is only 1 lens and 7 cm tall and there is a compound microscope where the image from objective lens is magnified again by the ocular lens used mainly for slides, in schools, labs, there are variations like brightfield illumination and darkfield illumination compound microscopes. Fluorescence microscope uses uv light and cells stained with fluorescent dyes. Confocal microscope uses fluorescence colors and laser light producing a 3d image. Scanning Acoustic microscope uses a computer and measures sound waves reflected back from object and it is used to study cells attached to a surface. Electron microscopes uses electrons instead of light and gives a greater resolution there are 3 kinds, TEM uses dead, ultrathin sliced specimens stained with heavy metal salt, SEM uses an electron gun that scans whole specimen and gives a 3d image and a Scanning-Probe microscope uses a metal probe to scan specimen and creates a 3d computer generated picture of specimen.

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12y ago

when there is a base of black so that organele can b appereqared

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10y ago

The two basic principles that are involved in using a microscope are magnification and observation. There are different types of microscopes which mainly include optical and electron.

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11y ago

1. At least two adjustable lenses

2. A light source to see the image

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10y ago

The two basic principles for using a microscope would make sure its on and slide is in place. Focusing in on the area of the slide using the large knob then the smaller knob.

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10y ago

The two basic principles that are involved using the microscope are magnification and observation. They are both done by examining an object through the lens of the microscope.

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10y ago

Basic technique in microscopy makes use of the differences in refractive index of cell structures.

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Q: What are the two basic principles involved in using a microscope?
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