What are the precautions for using a spectrophotometer?

Answer 1

Some simple precautions in the use of the spectrophotometer include:

• allowing the lamps and electronics to warm up
• using the correct wavelength
• wiping fingerprints and spilt sample off the outside of the cuvette before measuring
• carrying out the set-up procedure in the correct order
• performing calibration checks after set up
• closing the door to the cuvette compartment before reading the result
• cleaning up any spills inside the cuvette compartment
• ensuring that %T or transmission is used as appropriate.

Answer 2

1. Handle the spectrophotometer with care to avoid damage to its delicate components.
2. Keep the spectrophotometer in a clean environment to prevent dust or dirt from affecting readings.
3. Regularly calibrate the instrument using appropriate standards to ensure accurate measurements.
4. Follow the manufacturer's guidelines for maintenance and operation to prolong the spectrophotometer's lifespan.

Answer 3

spectrophotometre is sensitive so put away from hazardous chemicals

Answer 4

Making sure the spectrophotometer is free of dust .

The external surface of the tube must be free from fluid , to prevent false absorption