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Some drawbacks of gas chromatography include:

  1. The samples analysed are limited to those that are volatile or can be made volatile (reaction to form a volatile derivative).
  2. The samples must be thermally stable to prevent degradation when heated.
  3. Cannot be used to prepare samples for further analysis once separated.
  4. Problems can be encountered when injecting the sample:
  • It is difficult to measure and inject such small samples (approx 0.3microlitres) accurately without evapouration of the sample, for example.
  • The rubber seal through which the sample is injected may leak leading to loss of the sample.
  • Small pieces of the rubber septum may be adsorbed onto the column giving 'ghost peaks'.
  • The sample may be injected directly into the heated part of the injector so vapourisation may not occur (and the sample is lost).
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11y ago
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5mo ago

Some limitations of chromatography include difficulty in separating compounds with similar properties, limited resolving power for complex mixtures, and potential interference from impurities or contaminants in the samples. Additionally, chromatography techniques can be time-consuming and may require specialized equipment and expertise.

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7y ago

Difficult to reproduce, spots are often faint and not typically automated

spots are often faint, and TLC is difficult to reproduce. NOT typically automated

more points on liquid chromatography:

  • Over-large Spots
  • Uneven Advance of Solvent Front
  • Streaking
  • Spotting
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15y ago

Gas chromatography can only be used in cases where the substances can be vaporized without decomposing and where they can be vaporized at a reasonable temperature (i.e. not so hot that it destroys the column packing).

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10y ago

Advantages of HPLC • Speed (minutes) • High resolution • Sensitivity (ng to fg) • Reproducibility of +/- 1% • Accuracy• Automation Disadvantages of HPLC • Cost • Complexity • Low sensitivity for some compounds • Irreversibly adsorbed compounds not detected • Co-elution difficult to detect Isaac Kiplagat, MSc
High performance liquid chromatography (HPLC) is used to separate and identify compounds based on their polarity. The advantages of HPLC is that produces results within a few minutes. The results are high resolution and easily reproduced and tested.

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14y ago

That mainly depends on what kind of L.C. (Liquid Chromatography) is meant:

  • GLC = gas L.C. (Liquid as stationairy phase)
  • LLC = counter current liquids chromatography
  • SLC = Solid L.C. (Solid as stationairy phase, e.g. TLC = 'Thin layer' L.C. or Paper L.C. )
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14y ago

It's expensive to buy a GC/MS machine. The tubes you use for each sample are expensive. You need a trained operator. If you don't have your own machine you'll have to send the specimen off to be tested. And it's slower than most other ways of analyzing samples.

The other thing about GC/MS is either an advantage or a disadvantage depending on who you are. GC/MS is very, very accurate. If you smoke weed, being tested on an extremely accurate machine is a disadvantage. If you're trying to catch pot smokers, it's definitely an advantage.

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12y ago

the draw backs is that are that they will loos money

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11y ago

Chromatography can not be used in quantitative analyses and it doesn't allow the separation of complex mixtures. These are the limitations of chromatography.

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10y ago

An advantage of column chromatography is that is has both preparative as well as analytical applications. A disadvantage is the technical skill required to properly set up the column.

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What are the different types of chromatography?

The main types of chromatography include gas chromatography (GC), liquid chromatography (LC), thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). Each type of chromatography separates compounds based on their differing affinities for a mobile phase and a stationary phase.


What might be some limitations to paper Chromatography?

Some limitations of paper chromatography include limited resolution due to overlapping components, potential separation difficulties for compounds with similar properties, and the risk of sample contamination if the paper is not handled properly. Additionally, the process can be time-consuming and may require optimization for specific compounds or analytes.


How many types of Chromatography are there?

There are four main types of chromatography: gas chromatography (GC), liquid chromatography (LC), thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). Each type of chromatography has specific applications and uses in separating and analyzing chemical compounds.


How is paper chromatography used in everyday life?

it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.


What is grass chromatography?

Grass chromatography is a method used to separate and analyze the components of grass samples. It involves using a chromatography technique, such as thin-layer chromatography or gas chromatography, to separate the different compounds present in grass based on their chemical properties. Grass chromatography can be used to identify and quantify specific compounds like chlorophylls, carotenoids, and other pigments present in grass samples.

Related questions

What are the different types of chromatography?

The main types of chromatography include gas chromatography (GC), liquid chromatography (LC), thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). Each type of chromatography separates compounds based on their differing affinities for a mobile phase and a stationary phase.


What are 3 types of chromatography?

1. thin -layer chromatography 2. gas chromatography 3. liquid chromatography


What might be some limitations to paper Chromatography?

Some limitations of paper chromatography include limited resolution due to overlapping components, potential separation difficulties for compounds with similar properties, and the risk of sample contamination if the paper is not handled properly. Additionally, the process can be time-consuming and may require optimization for specific compounds or analytes.


How many types of Chromatography are there?

There are four main types of chromatography: gas chromatography (GC), liquid chromatography (LC), thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC). Each type of chromatography has specific applications and uses in separating and analyzing chemical compounds.


How is paper chromatography used in everyday life?

it can be used in everyday life by liquid chromatography, gas chromatography, thin-layer chromatographyand paper chromatography.


What is chromatography in science?

chromatography seperates substances from an object


What is grass chromatography?

Grass chromatography is a method used to separate and analyze the components of grass samples. It involves using a chromatography technique, such as thin-layer chromatography or gas chromatography, to separate the different compounds present in grass based on their chemical properties. Grass chromatography can be used to identify and quantify specific compounds like chlorophylls, carotenoids, and other pigments present in grass samples.


Is planar chromatography same as gas chromatography?

No, they are different techniques.


When was Journal of Chromatography A created?

Journal of Chromatography A was created in 1958.


When was Biomedical Chromatography created?

Biomedical Chromatography was created in 1986.


Are paper and thin layer chromatography types of planer chromatography?

yes.


When was Journal of Chromatography B created?

Journal of Chromatography B was created in 1958.