There is no single enzyme responsible for DNA duplication. But the most important ones are:
Helicase: it unwinds the DNA helix
RNA polymerase: adds the RNA primer
DNA polymerase: adds the complementary strand of DNA
Ligase: attaches the DNA fragments together
During DNA replication, hydrogen bonds between base pairs are broken by an enzyme called DNA helicase. This enzyme unwinds the double helix structure of DNA, separating the two strands. This allows for new complementary nucleotides to be added during the replication process.
The enzyme that copies DNA to make a molecule of RNA is called RNA polymerase. During the process of transcription, RNA polymerase binds to the DNA template strand and synthesizes a complementary RNA strand by adding ribonucleotides in the 5' to 3' direction. This process ultimately results in the formation of messenger RNA (mRNA), which carries genetic information from DNA to the ribosome for protein synthesis.
The enzyme responsible for the second step of DNA replication is called DNA polymerase. After the DNA strands are unwound by helicase, DNA polymerase synthesizes new DNA strands by adding nucleotides complementary to the template strands. This enzyme plays a crucial role in ensuring accurate and efficient DNA replication.
Topoisomerase is the enzyme that unwinds the DNA during replication. It binds to the DNA, and separates the double strands and form a replication fork. After which the primer bind to the start site, and DNA polymerase starts DNA synthesis.
The enzyme needed to make a DNA copy of RNA (including mRNA) is called reverse transcriptase. This enzyme is capable of synthesizing a complementary DNA strand from an RNA template, which is the first step in generating a cDNA (complementary DNA) library.
ligase
The enzyme
The enzyme responsible for cutting DNA molecules is called a restriction enzyme.
During DNA replication, hydrogen bonds between base pairs are broken by an enzyme called DNA helicase. This enzyme unwinds the double helix structure of DNA, separating the two strands. This allows for new complementary nucleotides to be added during the replication process.
The enzyme that copies DNA to make a molecule of RNA is called RNA polymerase. During the process of transcription, RNA polymerase binds to the DNA template strand and synthesizes a complementary RNA strand by adding ribonucleotides in the 5' to 3' direction. This process ultimately results in the formation of messenger RNA (mRNA), which carries genetic information from DNA to the ribosome for protein synthesis.
The enzyme that cuts DNA is called a restriction enzyme, while the enzyme that seals DNA is called DNA ligase. Restriction enzymes cut DNA at specific sequences, creating breaks in the DNA strands, while DNA ligase seals these breaks by catalyzing the formation of phosphodiester bonds between the DNA fragments.
In DNA replication, DNA polymerase III is the enzyme which joins the DNA nucleotides together via phospodiester bonds.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.
DNA replication
DNA Replication :)
Scientists use an enzyme called restriction enzyme to cut genes out of strands of DNA.
The enzyme that removes RNA primers during DNA replication is called DNA polymerase I.
Such an enzyme is called a restriction endonuclease