There is no single enzyme responsible for DNA duplication. But the most important ones are:
Helicase: it unwinds the DNA helix
RNA polymerase: adds the RNA primer
DNA polymerase: adds the complementary strand of DNA
Ligase: attaches the DNA fragments together
During DNA replication, hydrogen bonds between base pairs are broken by an enzyme called DNA helicase. This enzyme unwinds the double helix structure of DNA, separating the two strands. This allows for new complementary nucleotides to be added during the replication process.
Enzymes called helicases are responsible for separating the two DNA strands during DNA replication. Helicases unwind the double helix structure by breaking the hydrogen bonds between the base pairs, allowing the DNA polymerase enzyme to access and copy each strand.
The enzyme needed to make a DNA copy of RNA (including mRNA) is called reverse transcriptase. This enzyme is capable of synthesizing a complementary DNA strand from an RNA template, which is the first step in generating a cDNA (complementary DNA) library.
mRNA copies the information.Process is called transcription.
replication. In this process, the two strands of the DNA molecule unwind and each strand serves as a template for the synthesis of a new complementary strand. This results in two identical copies of the original DNA molecule.
ligase
The enzyme
The enzyme responsible for cutting DNA molecules is called a restriction enzyme.
The enzyme that cuts DNA is called a restriction enzyme, while the enzyme that seals DNA is called DNA ligase. Restriction enzymes cut DNA at specific sequences, creating breaks in the DNA strands, while DNA ligase seals these breaks by catalyzing the formation of phosphodiester bonds between the DNA fragments.
During DNA replication, hydrogen bonds between base pairs are broken by an enzyme called DNA helicase. This enzyme unwinds the double helix structure of DNA, separating the two strands. This allows for new complementary nucleotides to be added during the replication process.
In DNA replication, DNA polymerase III is the enzyme which joins the DNA nucleotides together via phospodiester bonds.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.DNA Ligase is the enzyme that seals gaps in DNA during DNA Replication.
DNA Replication :)
DNA replication
Scientists use an enzyme called restriction enzyme to cut genes out of strands of DNA.
The enzyme that removes RNA primers during DNA replication is called DNA polymerase I.
This enzyme is called a restriction enzyme. They are commonly used in molecular biology to create DNA fragments with specific ends that can be ligated together.
DNA polymerase