high-power lens
First of all, one should never call it high power, it is morecommonly called the "high objective", yet that is not what this question is asking. To answer the question: You use the fine adjustment knob. This knob should be located near the coarse adjustment knob, on the opposite side of the microscope (at the same height as the coarse adjustment knob), or even as a separate knob protruding from the coarse adjustment knob. The fine adjustment knob is smaller in size. You should never, ever use the coarse adjustment knob under the high objective, you could scratch the microscope slide, cover slip, high objective lens, or in a worst case scenario break the high objective lens.
Turning the coarse objective knob downward can cause the objective lens to come in contact with the slide, potentially damaging both the lens and the slide. It is best to always start with the coarse knob in the upward position to prevent any accidental collisions.
Oculars are generally 10x, so I assume you're talking of the 40x objective (10x40=400, how total magnification is derived). The most important rule is NEVER touch the coarse focus adjustment, only the fine; you run the risk of breaking the specimen or the objective if you mash the two together. Other things to consider- high light level, make sure koehler illumination is established and adjust your field aperture to eliminate refringence.
We avoid using the coarse focus knob with the 40x lens because it can lead to a high risk of crashing the lens into the slide, potentially damaging both the slide and the objective lens. The coarse focus knob moves the stage significantly, which is suitable for lower magnifications, but at high power, even small movements can cause contact. Instead, the fine focus knob should be used to make precise adjustments for clear imaging.
Pointing a microscope mirror directly at the sun can cause intense light to be focused into the microscope, leading to potential injury or damage to the microscope due to excessive heat or light intensity. This can cause the microscope's optics to overheat and potentially damage the lenses or cause a fire hazard. It's important to avoid pointing any optical instrument towards the sun to prevent accidents.
First of all, one should never call it high power, it is morecommonly called the "high objective", yet that is not what this question is asking. To answer the question: You use the fine adjustment knob. This knob should be located near the coarse adjustment knob, on the opposite side of the microscope (at the same height as the coarse adjustment knob), or even as a separate knob protruding from the coarse adjustment knob. The fine adjustment knob is smaller in size. You should never, ever use the coarse adjustment knob under the high objective, you could scratch the microscope slide, cover slip, high objective lens, or in a worst case scenario break the high objective lens.
You should avoid using the coarse focusing knob under high power magnification as it moves the microscope stage too quickly, making it difficult to focus accurately. It is best to only use the fine focusing knob to make precise adjustments when working with high power objectives.
Using the coarse adjustment knob on high power can potentially damage the microscope or the slide being observed due to the high magnification and close proximity of the objective lens to the slide. It is better to make coarse adjustments on low power to avoid hitting the objective lens against the slide.
Using the coarse adjustment knob on high power may cause the objective lens to come in contact with the slide, potentially damaging both the lens and the slide. It can also lead to misalignment of the optics, resulting in blurry images and potential damage to the microscope.
Because the movement is too large and you can break the slide of you turn it the wrong way.
Using the coarse adjustment on higher power can risk damaging the lens or the slide because the distance between the lens and the slide is very small. It can also cause the objective lens to hit and break the coverslip or slide. Finer adjustments should be made with the fine adjustment knob when using higher magnifications to prevent this.
Using the coarse adjustment knob on high power can cause the objective lens to hit the slide, potentially shattering both the slide and the lens. It can also damage the specimen and disrupt the focus, leading to blurry images.
Using the coarse adjustment knob on high power can potentially cause damage to both the microscope and the slide. High power magnification requires a more delicate touch to focus precisely, and the coarse adjustment knob moves the stage too quickly for fine adjustments at this level. It is better to use the fine adjustment knob on high power for accurate focusing.
Some common microscope rules include always starting with the lowest magnification, focusing carefully using coarse and fine adjustment knobs, keeping the lenses clean, handling slides gently, and using the microscope in a well-lit area. Additionally, it's important to never force any parts, such as the stage or focusing mechanisms, and to always carry the microscope with two hands.
Using the course adjustment knob on high power can cause the microscope to move too quickly, potentially damaging the specimen or the objective lens. It is better to use the fine adjustment knob on high power for precise focusing.
Turning the coarse objective knob downward can cause the objective lens to come in contact with the slide, potentially damaging both the lens and the slide. It is best to always start with the coarse knob in the upward position to prevent any accidental collisions.
You should never touch the objective lenses or the eyepiece of a microscope with your fingers. These components are delicate and any smudges or dirt can affect the quality of the images.