high-power lens
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The coarse adjustment knob on a microscope should never be used with the high-power objective lens. This can cause damage to both the lens and the slide being viewed. Instead, it is best used with the low-power or scanning objective lens for initial focusing.
First of all, one should never call it high power, it is morecommonly called the "high objective", yet that is not what this question is asking. To answer the question: You use the fine adjustment knob. This knob should be located near the coarse adjustment knob, on the opposite side of the microscope (at the same height as the coarse adjustment knob), or even as a separate knob protruding from the coarse adjustment knob. The fine adjustment knob is smaller in size. You should never, ever use the coarse adjustment knob under the high objective, you could scratch the microscope slide, cover slip, high objective lens, or in a worst case scenario break the high objective lens.
Turning the coarse objective knob downward can cause the objective lens to come in contact with the slide, potentially damaging both the lens and the slide. It is best to always start with the coarse knob in the upward position to prevent any accidental collisions.
To use a microscope at 400x magnification, start by placing the slide on the stage and securing it with the stage clips. Use the coarse focus knob to bring the specimen into view, then fine-tune the focus using the fine focus knob. Adjust the light intensity for optimal visibility, and remember to always handle the microscope with care to avoid damaging the lenses or stage.
Pointing a microscope mirror directly at the sun can cause intense light to be focused into the microscope, leading to potential injury or damage to the microscope due to excessive heat or light intensity. This can cause the microscope's optics to overheat and potentially damage the lenses or cause a fire hazard. It's important to avoid pointing any optical instrument towards the sun to prevent accidents.
Never visible to naked eye.Visible to light microscope during cell division