0
No. safranin is the classic stain used in gram staining. Concentrated Carbol Fushin is mainly used for the ZN staining procedure to stain organisms such as Vibrio cholerae and Cryptosporidium. Diluted Carbol Fushin can however be used as a replacement counterstain for Safranin in the gram stain.
Still curious? Ask our experts.
Chat with our AI personalities
Ross
Rene
Jordan
Add your answer:
Earn +20 pts
What is the purpose of heating slide with carbol fuchsin?
Heating the slide with carbol fuchsin helps to penetrate the bacterial cell wall and enhance the staining process. This allows the dye to better adhere to the bacterial cells, making them easier to visualize under the microscope.
What is the effect of heating the smear flooded with a carbol fuchsin stain?
Heating the smear flooded with carbol fuchsin stain helps in the penetration of the stain into the bacterial cell wall by softening the cell wall and making it more permeable. This process is important for the retention of the stain during the subsequent decolorization step in the staining process.
What is Carbol fuchsin used for?
Carbol fuchsin is a histological stain used in microbiology to detect acid-fast bacteria like Mycobacterium tuberculosis. It is commonly used in the Ziehl-Neelsen staining technique, where acid-fast bacteria retain the stain even when washed with acid-alcohol. This property helps in identifying these bacteria under the microscope.
Why should the the slide be flooded with a strong carbol fuschin?
Flooding the slide with strong carbol fuchsin helps in staining the mycobacteria in acid-fast staining techniques by allowing the stain to penetrate the mycolic acid layer in the cell wall. This improves the sensitivity of detecting acid-fast bacilli in the sample, making them more visible under the microscope. Additionally, the carbol fuchsin helps in differentiating acid-fast bacteria from other bacteria that may be present in the sample.
Which color is common to both the gram stain and the acid-fast stain?
The color common to both the gram stain and the acid-fast stain is red/pink. In the gram stain, Gram-negative bacteria appear red or pink after staining with safranin, while in the acid-fast stain, acid-fast bacteria such as Mycobacterium species retain the red/pink color of carbol fuchsin despite decolorization with acid-alcohol.
