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P. aeruginosa is not alpha hemolytic. Hemolysis is used mainly for the identification of Streptococci and can be used for Staphylococci. P. aeruginosa can be mistaken for being alpha hemolytic because it produces a green diffusable pigment. This pigment is especially visible if grown in media with little to no pH dye indicators such as SIM tubes, nutrient broths (undisturbed), API strips, etc.

A good way to tell the difference between diffusable pigment production and hemolysis is to look at the agar surrounding isolated colonies. Diffusable pigments should be alot more visible in areas with higher concentrations of bacteria (quadrant 1) and may not be seen around isolated colonies of 24 hour cultures. Hemolysis should be readily seen around isolated colonies regardless of incubation time.

If left long enough, P. aeruginosa will eventually clear all the RBC in the agar around the colonies (beta hemolysis).

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1mo ago

No, Pseudomonas aeruginosa is not considered alpha hemolytic. This bacterium is typically non-hemolytic, meaning it does not cause the breakdown of red blood cells.

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How can you differentiate the pigment of p aeruginosa on pseudomonas agar P from the pigment of E coli on MacConkey agar without referring to the colors?

It's not easy to differentiate the pigment of p aeruginosa on pseudomonas agar P from the pigment of E. coli on MacConkey agar without referring to the colors, but it can be done based on the structure of the p aeruginosa.


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