using uv-visible spectrophotometer
concentration vs absorbance is plotted and the maximum absorbance of the drug is lambda max of the drug. then after it will decrease. still if needed clarification, refer beer lambert"s law
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To calculate lambda max (λ max), generally used in spectrophotometry, you can use the formula: λ max = 1 / εmax, where εmax is the molar absorptivity (extinction coefficient) of the compound at its maximum absorbance wavelength. The λ max represents the wavelength at which the compound absorbs light most strongly.
To calculate the lambda max of alicyclic chromophores, you typically need to use computational methods like quantum chemical calculations or software tools like Gaussian or ORCA. These methods involve solving the Schrödinger equation to determine the electronic transition energies. The lambda max represents the wavelength at which the absorption of light is at its maximum for a specific alicyclic compound.
The lambda max is 510 nm.
The lambda max of S-adenosyl-methionine in UV spectrum is around 260-265 nm.
The UV-visible absorption spectrum of tapentadol typically shows a lambda max wavelength around 274-276 nm. This is the wavelength at which tapentadol exhibits maximum absorbance when exposed to UV light.
The lambda max of paracetamol in methanol is around 243 nm, while the lambda max of acetanilide in methanol is around 250 nm. These values represent the wavelengths at which the substances absorb light most strongly in a spectrophotometric analysis.