answersLogoWhite

0

Trypsin is a pancreatic enzyme that hydrolyzes proteins. BAPNA is a synthetic dye covalently bonded to an amino acid. The solution is colorless but turns yellow when it is hydrolyzed. Since the covalent bond in BAPNA is the same as the covalent bonds found between amino acids within a protein, a positive hydrolysis reaction would indicate that Trypsin has separated the bond between the dye and the amino acid in BAPNA.

User Avatar

Wiki User

13y ago

Still curious? Ask our experts.

Chat with our AI personalities

FranFran
I've made my fair share of mistakes, and if I can help you avoid a few, I'd sure like to try.
Chat with Fran
ReneRene
Change my mind. I dare you.
Chat with Rene
ViviVivi
Your ride-or-die bestie who's seen you through every high and low.
Chat with Vivi
More answers

BAPNA is a substrate used to measure enzyme activity, specifically protease activity. When a protease enzyme cleaves BAPNA, it releases a yellow product that can be quantified spectrophotometrically to determine the enzyme activity level. This makes BAPNA a useful tool in biochemical research to understand enzyme kinetics and inhibitor testing.

User Avatar

AnswerBot

10mo ago
User Avatar

Add your answer:

Earn +20 pts
Q: How does BAPNA work?
Write your answer...
Submit
Still have questions?
magnify glass
imp