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Trypsin is a pancreatic enzyme that hydrolyzes proteins. BAPNA is a synthetic dye covalently bonded to an amino acid. The solution is colorless but turns yellow when it is hydrolyzed. Since the covalent bond in BAPNA is the same as the covalent bonds found between amino acids within a protein, a positive hydrolysis reaction would indicate that Trypsin has separated the bond between the dye and the amino acid in BAPNA.

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13y ago

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BAPNA is a substrate used to measure enzyme activity, specifically protease activity. When a protease enzyme cleaves BAPNA, it releases a yellow product that can be quantified spectrophotometrically to determine the enzyme activity level. This makes BAPNA a useful tool in biochemical research to understand enzyme kinetics and inhibitor testing.

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11mo ago
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Q: How does BAPNA work?
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