They way that you prepare an indicator using and onion and beetroot depends on what type of indicator you want to prepare (i.e.: Litmus, pH, redox or other). Generally, you will cut pieces off of the onion and beetroot and put them each in separate flasks adding ethanol or other liquid you want to use for your indicator project.
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To prepare an indicator using onion and beetroot, you can grind or blend each separately with water to extract their pigments. The red pigment from beetroot will be acidic (pH < 7) and turn yellow in basic solutions (pH > 7), while the pigment from onion will be yellow in acidic solutions and red in basic solutions. These can be used to test the acidity or alkalinity of a solution by observing the color change.
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The juice of red beetroot change the color from red to yellow at a pH greater than 10.
One alternative to using red cabbage for a pH indicator is to use turmeric powder. Simply mix a small amount of turmeric powder with water to create a yellow solution. This solution will turn red in acidic conditions and green in basic conditions.
To locate a vacuole in an onion cell using a light microscope, first prepare a thin section of the onion tissue. Then, place the slide on the microscope stage and focus on the onion cells using low magnification. Look for large, clear spaces within the cell that might contain the vacuole, and adjust the focus to visualize it more clearly. The vacuole will appear as a membrane-bound organelle surrounded by cytoplasm.
To prepare alcian blue pH 1.0 solution, dissolve alcian blue powder in an acidic solution with a pH of 1.0, such as hydrochloric acid or formic acid. Adjust the pH of the solution using a pH meter or indicator strips to ensure it reaches the desired pH before use.
using a pH meter or pH indicator strips, which change color depending on the acidity or alkalinity of the substance. Another method is using universal indicator solution that changes color at different pH levels, allowing you to estimate the pH of the substance based on the color observed.