you can get methylene blue powder from a scientific store, it comes in powdered form. its pretty soluble in water and alcohol etc. the stain is made by dissolving an appropriate amount on methylene blue in a solvent, e.g for 0.1 dissolve 0.1% gram of methylene blue in 100 gram water, for 9% dissolve 9 grams
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To make a methylene blue solution, you can dissolve methylene blue powder in water at a desired concentration. The typical concentrations range from 0.1% to 1%. Make sure to use proper safety precautions, such as wearing gloves and protective eyewear, as methylene blue can stain skin and clothing.
Making a methylene blue stock solution is fairly easy. This makes 100 ml. Store at room temperature. Dissolve 0.2 g methylene blue-trihydrate (molar mass 373.9 g/mol) in 100 ml of DI water.
Adding methylene blue to sugar water solution.
If methylene blue is blue, it means that the compound is in its oxidized state (methylene blue) and has accepted electrons. Methylene blue can exist in both oxidized (blue) and reduced (colorless) forms depending on its redox state.
A student can use a stain called methylene blue to make nuclei more visible under a microscope. Methylene blue is commonly used in biology and histology to stain cells and highlight structures like nuclei.
Adding methylene blue to a slide will stain animal cells and make the nuclei more visible.
Methylene blue can inhibit the growth and reproduction of paramecium by disrupting cellular respiration and other metabolic processes. It interferes with the electron transport chain in mitochondria, affecting energy production in the cell and ultimately leading to its death.