Bacterial endospores can be used as indicators of sterilization efficiency because of their ability to enable bacteria to lie dormant for extended periods. If they are present then the sterilization is not efficient to kill them and thus the bacteria.
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Bacterial endospores are highly resistant to heat and chemicals, making them a good indicator for sterilization efficiency. By exposing test samples containing endospores to the sterilization process, the survival or destruction of the endospores can indicate whether the process was effective in killing all microbial life, including more fragile organisms. If endospores survive, it suggests that the sterilization process was not completely effective.
An indicator can become contaminated through exposure to environmental sources, improper handling, or contact with contaminated surfaces or materials. This can include inadequate sterilization practices, cross-contamination from other substances or samples, or exposure to air, water, or surfaces that contain contaminants.
Measuring bacterial growth at 600nm is a commonly used method because it corresponds well with bacterial cell density and is a quick and convenient way to monitor growth in real-time. The wavelength of 600nm falls within the range where bacterial cells absorb light due to their pigments like chlorophyll, carotenoids, and cytochromes, making it a suitable indicator of cell growth.
Watt indicator mechanisms are commonly used in steam engines to measure the power output of the engine in watts. This helps engineers monitor the efficiency and performance of the engine and make adjustments as needed. Watt indicator mechanisms typically consist of a pressure gauge and a crank mechanism to measure the pressure and power output of the steam engine.
Bacterial plasmolysis is the process in which a bacterial cell loses water and shrinks due to being placed in a hypertonic environment. This leads to the cell membrane pulling away from the cell wall, causing the cell to shrink and potentially die.
A bank-and-turn indicator is an instrument in an aircraft cockpit which incorporates the functions of a bank indicator and a turn indicator.