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Chromatography separates parts of a solution based on their different affinities for the mobile phase (solvent) and the stationary phase (solid support). Different compounds will interact differently with the stationary phase, causing them to move at different rates through the system and thus separate. This separation is based on factors such as polarity, size, and charge of the molecules.
Chromatography can still separate components in a non-colored solution based on their different chemical properties such as size, polarity, or charge. For example, in gas chromatography, compounds can be separated based on their boiling points and in size exclusion chromatography, molecules are separated by size. By utilizing these principles, chromatography can successfully separate and analyze components of non-colored solutions.
mixture of enantiomers can be separated by HPLC
two liquids having different solubility are separated by chromography.
Yes, a substance can be separated into physical parts through differences in physical properties. Techniques such as distillation, filtration, and chromatography are commonly used to separate mixtures based on differences in properties such as boiling point, solubility, or particle size.
No, compounds do not need to be colored to be separated by chromatography. Chromatography can also separate compounds based on differences in their chemical properties, such as polarity or size. The separated compounds may not be visible to the naked eye if they are colorless, but they can still be detected using other analytical techniques.