A mutation in DNA yields mutated mRNA (via transcription) which yields mutated proteins (via translation). Mutated proteins contain different amino acids (whether it be in a different order, shorter, longer, or missense).
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From DNA to protein, the series of events involves transcription, where the DNA sequence is copied into a messenger RNA (mRNA) molecule. This mRNA is then translated by ribosomes into a specific sequence of amino acids, forming a protein through the process of translation.
The Electromobility Shift Assay (EMSA) is a biochemical technique used to detect protein-DNA interactions. It involves separating DNA-protein complexes from free DNA through gel electrophoresis, allowing visualization of the binding events. EMSA is commonly used in molecular biology and biochemistry to study transcription factor binding, DNA-protein interactions, and related regulatory mechanisms.
DNA --> RNA --> Proteins -----------------------------------------That simple.
DNA tells a ribosome how to assemble a protein.
Transcription is the process that involves RNA polymerase. During transcription, RNA polymerase binds to a DNA template and synthesizes a complementary RNA molecule. This RNA molecule serves as a template for protein synthesis.
DNA synthesis produces DNA, not proteins. In DNA synthesis, new strands of DNA are produced by copying the existing DNA template. On the other hand, protein synthesis involves using the information encoded in DNA to produce proteins through the processes of transcription and translation.