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What is Electromobility shift assay?
One method for detecting DNA-protein interaction. EMSA rely on the ability of proteins to bind radiolabels DNA fragments in vitro. Once binding occurs, DNA-protein complexes can be separated from unbound DNA fragments by nondenaturing polyacrylamide gel electroforesis. In general the larger the protein or protein complex bound to the DNA, the greater the extend of retardation of mobility of the radiolabeled DNA fragment. The resulting protein-DNA complexes are visualized by autoradiography.Methods in muscle biology, Charles P. Emerson, H. Lee Sweeney.
In gene expression and protein synthesis information flows from?
DNA --> RNA --> Proteins -----------------------------------------That simple.
What tells ribosomes how to assemble a protein?
DNA tells a ribosome how to assemble a protein.
What process involves RNA polymerase?
Transcription is the process that involves RNA polymerase. During transcription, RNA polymerase binds to a DNA template and synthesizes a complementary RNA molecule. This RNA molecule serves as a template for protein synthesis.
Why DNA synthesis only protein?
DNA synthesis produces DNA, not proteins. In DNA synthesis, new strands of DNA are produced by copying the existing DNA template. On the other hand, protein synthesis involves using the information encoded in DNA to produce proteins through the processes of transcription and translation.
The sequence of events in the process of protein synthesis is usually?
The sequence of events in the process of protein synthesis involves transcription, where a messenger RNA (mRNA) is synthesized from a DNA template in the nucleus, and translation, where the mRNA is decoded by ribosomes to assemble amino acids into a protein. Additionally, during translation, transfer RNA (tRNA) molecules bring the corresponding amino acids to the ribosome based on the mRNA codon sequence.
How does chromatin immunoprecipitation work to identify protein-DNA interactions?
Chromatin immunoprecipitation (ChIP) is a technique used to study protein-DNA interactions. It involves cross-linking proteins to DNA, breaking the DNA into small fragments, and then using an antibody to pull down the protein of interest along with any DNA it is bound to. The DNA fragments can then be analyzed to identify the specific regions of the genome where the protein is interacting with DNA.
What is Electromobility shift assay?
One method for detecting DNA-protein interaction. EMSA rely on the ability of proteins to bind radiolabels DNA fragments in vitro. Once binding occurs, DNA-protein complexes can be separated from unbound DNA fragments by nondenaturing polyacrylamide gel electroforesis. In general the larger the protein or protein complex bound to the DNA, the greater the extend of retardation of mobility of the radiolabeled DNA fragment. The resulting protein-DNA complexes are visualized by autoradiography.Methods in muscle biology, Charles P. Emerson, H. Lee Sweeney.
What is the correct sequence of events that occur in a PCR reaction?
In a PCR reaction, the correct sequence of events is denaturation, annealing, and extension. Denaturation involves heating the DNA to separate the strands. Annealing involves cooling the reaction so primers can bind to the DNA. Extension involves DNA polymerase synthesizing a new strand of DNA using the primers as templates.
What is the process by which the expressed sequence of DNA codes for a protein?
The process by which the expressed sequence of DNA codes for a protein is called protein synthesis. This process involves two main steps: transcription, where the DNA sequence is copied into a messenger RNA (mRNA) molecule, and translation, where the mRNA is used as a template to assemble amino acids into a protein.
A section of DNA that codes for one protein?
A piece of DNA that codes for a particular protein is called a gene.
In gene expression and protein synthesis information flows from?
DNA --> RNA --> Proteins -----------------------------------------That simple.
What is the portion of the DNA molecule that contains the information for making 1 protein?
The portion of the DNA molecule that contains the information for making one protein is called a gene. Genes are specific sequences of DNA that encode instructions for creating a specific protein. The process of making a protein from a gene involves transcription of the gene into messenger RNA (mRNA) and translation of the mRNA into the corresponding protein by ribosomes.
Is DNA polymerase a protein?
Yes, DNA polymerase is a protein.
What is a length of DNA that codes for a protein?
A length of DNA that codes for a protein is called a gene. Genes contain the instructions for making specific proteins through a process called gene expression. This process involves transcription of the gene into messenger RNA (mRNA) and translation of mRNA into protein.
What tells ribosomes how to assemble a protein?
DNA tells a ribosome how to assemble a protein.
How did the Hershey and Chase experiment produce evidence that DNA and not protein is the hereditary material and viruses?
They found that all of the viral DNA and little of the protein had entered E. coli cells. Then they concluded that DNA is the hereditary molecule in viruses.
