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Polyclonal antibodies
Monoclonal antibodies
Inexpensive to produce
Expensive to produce
Technology required is low
High technology required
Skills required are low
Training is required for the technology use
Time scale is short
Time scale is long for hybridomas
Produces large amounts of non specific antibodies
Can produce large amounts of specific antibodies but may be too specific
Recognizes multiple epitopes on any one antigen
Recognizes only one epitope on an antigen
Can be batch to batch variability
Once a hybridoma is made it is a constant and renewable source and all batches will be identical
Monoclonal antibodies are derived from a single clone of B cells and therefore are identical in structure and specificity, targeting a single epitope on an antigen. Polyclonal antibodies, on the other hand, are produced by multiple clones of B cells and recognize multiple epitopes on an antigen, resulting in a mixture of antibodies with different specificities.
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What bond is antibody molecule held together by?
Antibody molecules are held together by disulfide bonds, which are covalent bonds formed between sulfur atoms in the amino acid cysteine. These bonds help maintain the structure and stability of the antibody molecule.
What is the main difference between a coomassie stain and a western blot?
A Coomassie stain is used to visualize total protein in a sample, while a western blot is used to detect specific proteins through antibody binding. Western blots are more specific and sensitive compared to Coomassie staining, as they rely on target-specific antibodies for detection.
What is the difference between 'collenchyma' and 'chlorenchyma'?
Difference between collenchyma and chlorenchyma
Difference in a charge between two electrodes in a cell causes a ----between electrodes?
Potential difference
What is the difference between og soluble and insoluble?
what is the difference between pf soluble and insoluble
What is the different between polyclonal antibody and antiserum?
polyclonal antobody is the antibody produced for many or non specific antigens but antiserum is the antibody for a specific antigen
What are the key differences between monoclonal antibodies and polyclonal antibodies in terms of their specificity and production methods?
Monoclonal antibodies are highly specific, targeting a single antigen, while polyclonal antibodies can target multiple antigens. Monoclonal antibodies are produced from a single clone of cells, resulting in uniformity, while polyclonal antibodies are produced from multiple clones of cells, leading to variability.
What are the key differences between polyclonal antibodies and monoclonal antibodies in terms of their specificity and production methods?
Polyclonal antibodies are produced by multiple B cells and are a mix of antibodies that target different epitopes on an antigen, resulting in broader specificity. Monoclonal antibodies are produced by a single B cell clone and target a single epitope on an antigen, leading to higher specificity. Polyclonal antibodies are typically produced by immunizing animals, while monoclonal antibodies are generated through hybridoma technology or recombinant DNA technology.
Are chickenpox antibody and shingles antibody the same?
Chickenpox and shingles result from the same virus, and generate the same antibodies. There is no difference between chickenpox antibody and shingles antibody, and there is only one test (varicella virus antibody) for both.
What is the connection between rheumatoid arthritis and GM-CSF?
For information on this subject go to the related link (MedImmune Initiates First Clinical Trial of Monoclonal Antibody Targeting GM-CSFR in Patients with Rheumatoid Arthritis) Below
What is the difference between an antibody and a B cell receptor?
Antibodies lack a transmembrane domain.
What Is the difference between antigen binding site and paratope?
Antigen binding site or epitope is a part of an antigen that is recognized by the antibody. Paratope is a part of an antibody that binds on epitope.
What is the difference between opsonization and neutralization?
Opsonization involves an antibody binding to a pathogen itself while neutralizing invovles an antibody binding to a toxin, AKA something the pathogen produces.
What is the difference between alloimmunization and isoimmunization?
the autoimmune reaction is the reaction between the antibody and antigene when both come from the same body but the isoimmune reaction is between the antigene and antibody produced for the same antigene ( the same according to the type but not the immunity )
What happens when the epidermis and the dermis are destroyed?
difference between an antibody and an antigen
What is the difference between antibody and immunogloulin?
Antibodies are secreted (plasma cells), while immunoglobulins are membrane-bound (naive B cells).
What is the difference between heavy chain and light chain in the context of antibody structure?
In the context of antibody structure, heavy chains are larger and provide structural support, while light chains are smaller and help with antigen binding.
