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Are SDS and SDS-plus bits the same?
The short answer to your question is "yes". I found myself researching the same question a few days ago and found that the real difference is between SDS/SDS Plus and SDS Max. I don't recall the exact dimension now, so I won't try to quote it, but the Max is a larger size. The answer I found was enough to tell me I used SDS (SDS Plus), and those were the bits I needed to buy. Once I knew that, I didn't need to remember the size of SDS Max...they were too big for my drill. Last point, SDS Plus is sometimes shortened to SDS+.
Why does Escherichia coli suspention become more viscous after the addition of SDS?
SDS (sodium dodecyl sulfate) disrupts the cell membrane of Escherichia coli, releasing cellular components like DNA and proteins into the suspension. These cellular components can aggregate and interact with SDS molecules, increasing the overall viscosity of the suspension due to the formation of complexes and networks.
What is the advantage of adding SDS to gel electrophoresis?
Adding SDS to gel electrophoresis helps denature proteins by breaking down their native structure and coating them with negative charges, allowing for more uniform migration based on size. This results in better separation of protein bands in the gel based on their molecular weight.
What water do you use in an autoclave?
Demineralised water should best be used in an autoclave.
Can glucose solution be autoclave?
Yes, glucose solutions can be autoclaved to sterilize them. However, it's important to note that high temperature and pressure during autoclaving can cause caramelization or degradation of the glucose solution, so care should be taken to prevent overheating.
