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1. DNA is negatively charged by placing the sample wells closest to the negative electrode we can give the DNA more room to spread out in accordance to its size and charge. The amount the DNA moves from the well is directly linked to its charge and size. Since DNA is only negatively charged leaving space for the DNA to move in the positive direction would be a waste of space because it is not possible.

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Q: Why is it important to position the sample wells near the negative electrode?
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