If you are referring to the Well of Souls scene in the movie, yes, the snakes used were real. The set had 2000 snakes at first, but Steven Spielberg felt it wasn't scary enough. So, an additional 4500 snakes were shipped in for the set.
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Going by film release order, it was Raiders of the Lost Ark, rebranded as it were to Indiana Jones and the Raiders of the Lost Ark. If you are going by chronological order, then it is Indiana Jones and the Temple of Doom.
1. Indiana Jones and the Raiders of the Lost Ark 2. Indiana Jones and the Temple of Doom 3. Indiana Jones and The Last Crusade 4. Indiana Jones Kingdom of the Crystal Skull
Indiana Jones and the Raiders of the Lost Ark and Indiana Jones and the Last Crusade.
no
That's in the opening sequence for the first movie, the Raiders of the Lost Ark.