EDTA
Sodium Fluoride
EDTA in tubes has been the source of more carryover problems than any other additive. Heparin causes the least interference in tests other than coagulation tests because it also occurs in blood naturally but may affect tests done on a clotted tube (SST) if blood is poured over.
Foundation: Glycolysis isn't totally or typically restrained by the glucose additive right now being used, with glucose esteems falling by as much as 0.5 mmol/L during a 2-4-h period after example assortment. Quick centrifugation of all examples is likewise unfeasible and in this way misdiagnosis of sickness can happen, particularly if more accentuation is being set on fasting glucose for the determination of Diabetes.
Techniques: Glycolysis at room temperature was assessed after some time utilizing glyceraldehyde alone just as related to standard antiglycolytic specialists.
Results: Glyceraldehyde alone doesn't restrain glycolysis totally. The mix of 11 mmol/L glyceraldehyde, 119 mmol/L sodium fluoride and 21.7 mmol/L potassium oxalate gave the best antiglycolytic results. The glucose esteems estimated in examples put away at room temperature for 48 h was the same as those deliberate in examples centrifuged following venepuncture and this is clinically better than routinely utilized sodium fluoride and potassium oxalate.
End: Plasma glucose fixations acquired from blood gathered into tubes containing glyceraldehyde, sodium fluoride and potassium oxalate will all the more intently mirror those of the patient at venepuncture.
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sodium fluoride
Its purpose is to inhibit the metabolism of glucose by cells in a specimen of blood.
The gray-topped tube, also known as a fluoride tube, contains an antiglycolytic agent such as potassium oxalate or sodium fluoride. This tube is used to preserve glucose levels in a blood sample by inhibiting glycolysis, which is the breakdown of glucose by cells.
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