In the Gospels.
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The combined words "Virgin Mary" are not mentioned in the Christian bible.
The name Mary was first mentioned in Matthew 1 dring the genealogy of Jesus. Mary was the virgin that gave birth to the Saviour. So she was technically mentioned in the old testament when they prophesied Jesus' coming.
The first time she is mentioned in the Bible is in Luke 8:3.
52 times
Approximately 54 times