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A UPLC (ultra-high performance liquid chromatography) is a variant of HPLC using columns with particle size <2 um (typically, 1.8 um), which provides significantly better separation than the traditional (5 um) columns and enables much faster analysis. Strictly speaking, "UPLC" is Waters Corporation trademark, but is often used as a name for the technique in general.
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What are the advantages of UPLC over HPLC?
UPLC (Ultra Performance Liquid Chromatography) typically provides faster analysis times, higher resolution, and improved sensitivity compared to traditional HPLC (High Performance Liquid Chromatography). UPLC systems use smaller particle sizes in stationary phases and higher pressures, leading to better separation efficiency and lower solvent consumption.
What is Difference Between HPLC UV detector Spectrophotometer uv detector?
HPLC UV detector is a component used in high-performance liquid chromatography (HPLC) to monitor eluent absorbance, while a spectrophotometer UV detector is a standalone instrument used to measure the absorption of light at different wavelengths. HPLC UV detectors are specifically tailored for chromatography applications, whereas spectrophotometer UV detectors are more versatile and used for various analytical purposes.
What is difference between high pressure liquid chromatography and high performance lequid chromatography?
High pressure liquid chromatography (HPLC) and high performance liquid chromatography (HPLC) are often used interchangeably. HPLC refers to modern liquid chromatography systems with high resolution and efficiency, while high pressure liquid chromatography specifically highlights the use of higher pressures in the system to improve separation and speed. Both terms generally refer to the same chromatographic technique.
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How do you use resolution factor in HPLC?
The resolution factor in HPLC is used to quantify the degree of separation between two adjacent peaks on a chromatogram. It is calculated by dividing the difference in retention times of the two peaks by the sum of their peak widths. A higher resolution factor indicates better separation between the peaks.
How do you distinguised np-hplc and rp-hplc?
NP-HPLC (normal phase HPLC) separates compounds based on their polarity, where the stationary phase is polar and the mobile phase is nonpolar. RP-HPLC (reverse phase HPLC) separates compounds based on their hydrophobicity, where the stationary phase is nonpolar and the mobile phase is polar. RP-HPLC is more commonly used due to its versatility and ability to handle a wider range of compounds.
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What is difference between isocratic and gradient hplc?
In isocratic HPLC, the mobile phase composition remains constant throughout the entire run, leading to constant elution times for all analytes. In gradient HPLC, the mobile phase composition is changed during the run, allowing for better separation of complex mixtures by adjusting the solvent strength over time.
What is difference bet GLC and HPLC?
GLC (Gas Liquid Chromatography) uses gas as the mobile phase and liquid as the stationary phase, while HPLC (High Performance Liquid Chromatography) uses liquid as the mobile phase and a solid or semi-solid stationary phase. GLC is mainly used for volatile compounds, while HPLC is more versatile and can analyze a broader range of compounds.
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
What is rs-hplc?
"RS-HPLC method" means "Related Substance HPLC Method".
