It was the treaty line in the Missouri Compromise. It determined the status of slavery in the unorganized Louisiana Territory. All land above it was free and all land below it was slave.
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Yes, it was agreed that all states admitted after the Missouri Compromise was signed above 36 30 line would be free.
36 degrees north and 30'
Missouri Compromise
Slavery was prohibited in land north of the 36-30 parallel
The Missouri Compromise of 1820