No, not really. In fact it was the hub of the ancient world . Food and other items from other places could be found in Rome. Sea ports were fairly close, travel was easy on the Roman roads, and the only major mountains were north of Rome. It sits in a flat plain and has good river access into Rome.
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During the early days of the ancient Roman civilization, Rome was positioned in the middle of the Italian peninsula. The close major civilizations of Carthage and the ancient Greek colonies in Southern Italy and Sicily were not a major threat. The Roman republic would not have a conflict with Carthage for several hundred more years. Powerful major eastern civilizations in the eastern part of the Mediterranean Sea, such as the Parthians, were isolated from early Rome.
The Mayans were geographically isolated from some civilizations at their time. Civilizations like Greece, Rome, Chinese, Indian, Egyptian, and Mesopotamian people were separated by the Atlantic ocean and at the time no one had the technology to cross it. But in South America there were Aztecs, Tapotec, Mixtec, Olmec, Toltec, and Teotihuacan and Inca populations.
yes the Chinese were isolated from other civilizations.
Because of its geography
Greece was not centered around a river valley.
The mountain ranges in Greece would create vallies where citys were built. Because the mountains were very hard to travel over they isolated the city to develope individully from the other citys making them city-states.