Brazil.
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It led Portuguese leaders to leave Europe and rule from the colony of Brazil. When Portugal became independent of the French Occupation in 1815, the Brazilians had become accustomed to having local leadership and this became a major issue leading to the War of Independence of Brazil in 1822.
the expansion of slavery
actually, slave trading is still an issue today. And not just with blacks, but of all races.
To tell European countries to leave the Americas alone(APEX)
an eight hour work day