heparin may be extrected along with DNA
DNA isolation is a based on the principle of purification. DNA samples are isolated through the use of physical and chemical methods. Friedrich Miescher conducted the first isolation of DNA in 1869.
Agarose is not used in DNA isolation. Agarose is used to prepare a gel in which DNA fragments can be separated based on size
phenol is used in order to remove protein impurities from the DNA to yield pure dna while chloroform prevents shearing of DNA during isolation.
70% ethnol is used for the dna isolation becuse it makes hydrogen bonding with the water molecules and makes DNA hydrophopic so that it pricipitated.
Gene sequencing and gene cloning
mgcl2 work as a cofactor and they enhance the enzymatic reaction..
Extraction Buffer is used to maintain pH of the solution.which prevents denaturation of DNA.
CTAB is a detergent used to denature proteins from samples. Once the protens have been denatures, the isolation of DNA from the non required waste materials can begin.
it act as as a cationic detergent for the isolation dna from the given sample
Sucrose performs the function of osmoregulation in the protocol of DNA isolation from blood
The DNA fragments comes from the method of DNA isolation.
to precipitate extracted DNA