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The purpose of any microbiological stain (gram or otherwise), is to enable visualization of features that would otherwise be clear/invisible in a sample. In this case, the darker crystal violet is used to stain the peptidoglycan cell walls of gram-positive bacteria. Washing the sample with too much ethanol (decolorizing too much) will remove too much stain, making it difficult or impossible to distinguish between gram-negative and gram-positive cells. Only the counterstain that is added after the ethanol wash (safranin, for example) will be visible.

(see related links for example descriptions of Gram staining)

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Q: What would happen if you decolorized your slide too much on a gram stain?
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Related questions

What would the slide look like under the microscope if methylene blue is used instead of nigrosin?

The stain would stain the cells rather than the background

What would you expect from a gram stain done on a slide that was heated too hot during the heat-fixed smear?

A Gram stain refers to a staining technique for the identification of bacteria. A Gram stain done on a slide that was heated too hot during the heat-fixed smear will destroy the cell wall of the bacteria.

What would happen if you slide down a slide with water on it?

youd go faster, but the slide would get sticky after a while and would give someone a wedgie or pull their pants down!

What will happen to food if there was no friction?

it would just slide away

What color are gram-negative organisms if you over-decolorized them?

If the Gram Stain is completed properly, gram positive should stain purple; however, if you over decolorize a gram positive organism, the organism will appear appear pink, which is a gram negative reaction. To summarize, if you over decolorize a gram positive organism it will show as a gram negative organism.

What is it called when water ruins wood?

i think it's called a water stain, it can happen to about everything, so i think it would be called a water stain on wood.

If Congo red is used instead of safranin in the gram stain technique what would happen?


What happen to the letters aj and k when you move the slide?

suppose you wanted to follow an organism that was moving to the right, which way would you move the slide?

What would happen if the step involving Gram's iodine was inadvertently left out of the Gram staining procedure?

Gram staining refers to separating bacterial species into two groups, gram-positive and gram-negative, which defines the physical and chemical properties. Skipping a step in this process could result in a mixed group of the bacterial species.

What is methylene blue use for when gram staining?

Methylene blue can be used to prepare slide for animal cells. For example, if you want to examine a piece of your cheek cell, you would take a piece of cotton wool and rub it on the inside of your cheek and the rub it on a microscope slide, the you add a little distilled water and then a drop of methylene blue. The final step you would take, is to cover the slide with a cover slip, then place it under the microscope.

What would happen if the slide had high friction?

It wouldnt be a slide. It would be a larger chair depending on the moleculer compounds involed in the changing plastyic compound. If it is a metal the mole ratio will cause the slide to become abnormally hard. 8====D

What would happen if a slide had high friction?

If a slide had high friction, it would not function well as a slide. Things would either slow down during their descent or would stop completely (if the coefficient of friction were sufficiently large).You would either stop in mid slide, burn your bottom,or shred the seat of your pants.