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What do you call a population of cells carrying a desired plasmid?
Wiki User
∙ 9y ago
A plasmid is a small molecule of DNA that replicate independently within the cell. A population of cells carrying a desired plasmid is called a clone.
Wiki User
∙ 9y ago
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A population of cells carrying a desired plasmid is called a?
Clone
What is a Self replicating DNA used to transmit a gene from one organism to another?
Self replicating DNA used to transmit a gene from one organism to another is a vector. A population of cells carrying a desired plasmid is called a clone.
How will you identify cells carrying the recombinant plasmid?
The transformants are selected for on agar containing an appropriate antibiotic. For example if your recombinant plasmid contains a kanamycin cassette, then only the cells containing the plasmid will grow on an agar plate containing kanamycin. PCR can then be performed on the colonies to ensure they contain your gene of interest on the plasmid.
What is found in only prokaryotic cells?
plasmid
What type of gene is used to distinguish bacteria that carry a plasmid containing foreign DNA from those that don' t?
The plasmid that contains foreign DNA is engineered to also carry an antibiotic resistance gene. This antibiotic resistance gene codes for a protein that is able to inactivate an antibiotic thus keeping the cell alive. In the absence of the antibiotic resistance gene, the cells would not survive when exposed to an antibiotic. After transfection (the process of inserting the plasmid carrying the foreign gene into cells), the cells are gown in media containing an antibiotic. Cells that contain the plasmid (and therefore contain the antibiotic resistance gene) are able to survive in this medium. Cells that do not contain the plasmid (and therefore lack the antibiotic resistance gene) do not survive in this medium. The process described above is called selection
When plasmids are used to transfer foreign DNA to other cells the plasmid is called?
When plasmids, are used to transfer foreign DNA to other cells the plasmid is called a
A single piece of coiled DNA is known as?
A coiled piece of DNA is called a plasmid, found in prokaryotic cells.
What enzyme is produced by cells transformed with plasmid lux that is not produced by cells transformed with pUC18?
Luciferase
What is the similarities and difference between the bacteria and human cells?
a human cells have DNA and bacteria has plasmid
Which feature is found most often in prokaryotic cells?
plasmid
What is a plasmid exchange?
Plasmid Exchange is the transfer of genetic material between bacteria through direct cell to cell contact, or through a bridge-like connection between the two cells.
How does a human insulin genes become part of a plasmid?
1. Scientists remove plasmids, small rings of DNA, from bacterial cells. 2. An enzyme cuts open the plasmid DNA. The same enzyme removes the human insulin gene from its chromosome. 3. The human insulin gene attaches the open ends of the plasmid to form a closed ring. 4. Some bacterial cells take up the plasmids that have the insulin gene. 5. When cells reproduce, the news cells will contain copies of the engineered plasmid. The foreign gene directs the cell to produce human insulin.
