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To properly collect a 24 hour urine - empty the bladder at some time that's easy to remember, like 8AM or the time you get up every day.
After emptying the bladder at that start time, collect all urine up to that same time the next day. At the same time the next day, empty the bladder one last time and save that urine also.
So, start with an empty bladder and end with an empty bladder.
Sometimes the collected urine has to be kept from light or refrigerated. If so, be sure to try to keep the collection container covered or cool.
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