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The Eosine Methylene Blue test is selective for only gram-negative bacteria. It is possible for gram-positive bacteria to grow, but it would barely grow. Staphylococcus are gram-positive spherical bacteria. So it would not grow on the Eosine methylene Blue Agar.

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Q: What are the EMB test results for Staphylococcus epidermidis?
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Will staph grow on EMB agar?

Staphylococcus bacteria typically do not grow well on Eosin Methylene Blue (EMB) agar. EMB agar is selective for gram-negative bacteria, particularly Enterobacteriaceae, and contains dyes that inhibit the growth of gram-positive bacteria like Staphylococcus.


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EMB agar is selective for Gram-negative bacteria and can differentiate between lactose fermenters and non-fermenters based on colony color. MacConkey agar is selective for Gram-negative bacteria and contains lactose and pH indicators to differentiate between lactose fermenters (pink colonies) and non-fermenters (colorless colonies).


EMB is a selective medium Is it also a defined or an undefined medium Why is that formulation desirable?

EMB (Eosin Methylene Blue) is a defined medium because the composition and exact amounts of each ingredient are known. This formulation is desirable for consistent and reproducible results in microbial culture experiments because researchers can control and understand the nutritional content of the medium.


Will pseudomona aeruginosa grow on Emb?

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Eosin methylene blue (EMB) agar is selective for gram-negative bacteria, especially those that can ferment lactose. Common bacteria that grow on EMB agar include Escherichia coli and Enterobacter aerogenes. These bacteria are differentiated by their ability to ferment lactose, producing colonies that are metallic green or dark purple on EMB agar.


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