The following are some advantages of an agar plate verses a slant tube:
1. Surface area- An agar plate has a much larger surface area:
a. Easier to isolate individual colonies using the streak-plate method.
i. Evaluate the colony shape, margin and elevation.
b. Can grow a larger number of cells.
2. Growth- An agar plate allows you to quantify the number of colonies on an agar plate, provided it is within the 30-300 range. Whereas the slant tube cannot quantify growth but only describes growth as none, slight, moderate, or large.
Slants are better suited than agar plates, because they can be capped, preventing the agar and the culture from drying out. The cap also prevents airborne contaminants from entering the slant. Also, slants take up less storage space than an agar plate.
Agar is made from vegetable matter - Gelatin is made from animal bones.
Advantages: 1. Counts only living cells 2. Standardized test - used worldwide Disadvantages: 1. 1-2 days of incubation 2. Melted, heated agar 3. Osmotic shock
The hockey stick is used to spread the microbial inoculum evenly across the agar surface in a spread plate method. By dragging the hockey stick back and forth over the agar surface, it helps to distribute the microbes in a consistent and uniform manner, promoting even colony growth.
An agar slant is when a test tube is filled with liquid agar and allowed to cool and harden at an angle (slant). Agar is mixed with other nutrients to provide a medium for which bacteria can grow on.
Previous answer: "because of infection" This person is obviously trying to be funny, the right word should be "Contamination", as for spread plate, the bacteria is more exposed to air as it is spread over the agar plate. Therefore, the result might not be accurate, as it might be contaminated. As for the pour plate method, the bacteria is in the agar itself, it is not exposed to air, thus, less risk of getting contaminated.
The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.
Instantaneous results. The primary advantage of the photographic plate over CCDs is image quality--an 8x10 plate carries a lot more image information than a CCD can capture.
Streak Plate:Pure colonies of bacterial or other microorganisms can be obtained on petri dishes by streak plate. The microbial mixture is transfered to the edge of an agar plate with an inoculating loop or swab and then streaked out over the surface in one of several patterns. After the first sector is streaked in dish, the inoculating loop is sterlized and an inoculum for the second sector is obtained from the first sector. The same is done for third and fourth sector. Thus this is a dilution process. Eventually, very few cells will be on inoculating loop, a single cells will drop from it as it is rubbed along the agar surface. These develop into seprate colonies.Pour Plate:Extensively used with procaryotes and fungi, a pour plate also can yield isolated colonies. The original sample is diluted several times to reduce the microbial population sufficiently to obtain separate colonies when plating. Then small volumes of several diluted samples are mixed with liquid agar that has been cooled to about 45oC and the mixture are poured immediately into sterile culture dishes. Most bacteria and fungi are not killed by a brief exposure to the warm agar. After the agar has hardened each cell is fixed in place and forms an individual colony.
There are many advantages of multi-plate clutches over single plate ones. They decrease the moment of inertia of the clutch and increase the amount of torque that is transmitted through increasing the frictional coefficient of the clutch plates. In addition, they decrease the amount of force needed to operate the clutch, enabling faster and smoother shifting.
Sources of error in viable plate counting include inaccurate dilutions leading to over or underestimation of colony forming units, uneven distribution of bacteria on the agar plate causing inaccurate colony counts, contamination from environmental sources impacting the results, and variability in the incubation conditions affecting bacterial growth rates.
The car began to slowly roll past the graveyard due to the imperceptible slant in the road. He's wearing his hat on a rakish slant.