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The following are some advantages of an agar plate verses a slant tube:
1. Surface area- An agar plate has a much larger surface area:
a. Easier to isolate individual colonies using the streak-plate method.
i. Evaluate the colony shape, margin and elevation.
b. Can grow a larger number of cells.
2. Growth- An agar plate allows you to quantify the number of colonies on an agar plate, provided it is within the 30-300 range. Whereas the slant tube cannot quantify growth but only describes growth as none, slight, moderate, or large.
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What is the advantage of using a nutrient agar plate over a nutrient agar slant?
Slants are better suited than agar plates, because they can be capped, preventing the agar and the culture from drying out. The cap also prevents airborne contaminants from entering the slant. Also, slants take up less storage space than an agar plate.
What are the advantages and disadvantages of pour plate method?
Advantages: 1. Counts only living cells 2. Standardized test - used worldwide Disadvantages: 1. 1-2 days of incubation 2. Melted, heated agar 3. Osmotic shock
What are 2 advantages agar has over gelatin for microbiological media?
Agar is made from vegetable matter - Gelatin is made from animal bones.
What is the function of hockey stick in spread plate method?
The hockey stick spreads the known concentration of bacteria evenly over the agar plate.
Use of agar slant?
An agar slant is when a test tube is filled with liquid agar and allowed to cool and harden at an angle (slant). Agar is mixed with other nutrients to provide a medium for which bacteria can grow on.
What is the purpose of a control agar plate?
A control in an agar plate is the left over spot where you have put no bacteria swabs on, that way when the bacterias have grown you can see if the bacterias spread onto the control, or if the control stayed disinfected.
What is the main advantage of pour-plate method over other methods of bacterial colony isolation?
Previous answer: "because of infection" This person is obviously trying to be funny, the right word should be "Contamination", as for spread plate, the bacteria is more exposed to air as it is spread over the agar plate. Therefore, the result might not be accurate, as it might be contaminated. As for the pour plate method, the bacteria is in the agar itself, it is not exposed to air, thus, less risk of getting contaminated.
Advantages of pour plate method over other methods of bacterial colony?
The purpose of the spread-plate technique is to grow and isolate colonies of bacteria. A sample of bacteria is transferred to the agar plate, an environment that provides nourishment for the bacteria to grow. The bacteria sample is applied to the agar plate which a special streaking technique that dilutes the amount of bacteria in each section of the agar plate continuously. This is because if you just swabbed the bacteria onto the plate with no special technique the colonies would grow very densely together and be difficult to study. The streaking technique gradually dilutes the amount of bacteria in each 'quadrant' of the plate, so the last quadrant should have small, isolated colonies that can be easily studied. The spread plate technique is also used for the eneumeration of aerobic microorganisms from the given sample. This can be done by serial diluting the samples, placing 0.1ml of the diluted sample in the middle of an agar plate and spreading the sample over the surface with a help of an L-rod. After the incubation rhe colonies can be counted.
What are the advantages of a CCD over a photographic plate?
Instantaneous results. The primary advantage of the photographic plate over CCDs is image quality--an 8x10 plate carries a lot more image information than a CCD can capture.
What would happen if plates were incubated for a month longer?
A lot depends on what is on the plates in the first place and what conditions you are incubating under. Probably the answer you are looking for is- the growth would be so much that the individual colonies would merge together. This would make it impossible to pick a single colony for further experimentation. If the plate contains a mixed sample consider the possibility of swarming/motile bacteria taking over the entire plate. If the plate is being cultured under special conditions consider if you technique is sufficently robust to maintain them for the whole time. For example cultures at high temperaters may desicate if measures are not taken to ensure humidity.
Advantages of multiplate clutch over single plate clutch?
There are many advantages of multi-plate clutches over single plate ones. They decrease the moment of inertia of the clutch and increase the amount of torque that is transmitted through increasing the frictional coefficient of the clutch plates. In addition, they decrease the amount of force needed to operate the clutch, enabling faster and smoother shifting.
Why does the streaking method you used to inoculate you plates result in isolated colonies?
In the streak plate technique, a loop is used to streak the mixed sample many times over the surface of a solid culture medium in a Petri plate. Theoretically, the process of streaking the loop repeatedly over the agar surface causes the bacteria to fall off the loop one by one and ultimately to be distributed over the agar surface, where each cell develops into a colony.
