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Does bacillus subtilis have a positive reaction for ONPG Test?
No, Bacillus subtilis does not have a positive reaction for the ONPG test. This test is used to detect the presence of β-galactosidase enzyme, which is not produced by Bacillus subtilis.
Why are some organisms positive in the ONPG Test but negative when tested for the ability to metabolize lactose?
In the ONPG test, organisms may be positive due to the presence of beta-galactosidase enzyme, which cleaves ONPG and produces a yellow color. However, in the lactose fermentation test, some organisms may lack other necessary enzymes or transport systems to fully metabolize lactose, resulting in a negative result despite having beta-galactosidase.
Why cant the ONPG test be substituted for the determination of lactose fermentation?
ONPG test detects only presence of beta galactosidase enzyme whereas lactose fermentation requires the presence of permease as well as beta galactosidase enzyme.
Biochemical tests for Salmonella and Shigella?
urease - negative ONPG - positive Lysine - positive
Motility is best seen with a?
Wet mount, hanging drop slide, or motility tube (SIMS, ONPG-PAM)
Why incubate ONPG?
too see transcription and translation speed - u plot it against time (A400 - A550*1.65) / 0.0075
Are microbes harmful?
All Microbes are not harmful. All virus are harmful. Some bacteria are harmful
Are rodent harmful to us?
some are harmful but some are not harmful
Can shigella sonnei be a lactose non fermenter My tests are as followed TSI KA Methyl red VP - citrate - urea - NO3 degraded to NO2 H2S - motility -. all points to shigella but it's nonferm?
S.sonnei is not a true lactose fermenting bacteria (see.. http://www.pubmedcentral.nih.gov/picrender.fcgi?artid=271578&blobtype=pdf)But is a delayed lactose fermenter as it does not have the enzyme permease to transport lactose through its cell wall.However an ONPG lactose fermenting test does not require permease to allow the bacterium to ferment lactose so in an ONPG test the shigella sonnei will appear as lactose fermenting positive.Hope this helps!!2nd year BMS student
Are mantises harmful?
No they are not harmful.
What are the comparative and superlative forms of harmful?
more harmful, most harmful
Are lions harmful or useful?
lion are useful and harmful
