as such its very vague. because it depends on part to part if the upper limb. loss of blood supply to any of the body organs can lead to avascular necrosis i.e death of the tissue due to loss of blood supply. some parts are very sensitive to this as necrose immidiately after loss of blood supply whereas some parts can resist the stress and necrose after some time. but its generally said that that the avascularized part's blood supply should be fixed within 5 to 8 hours(for muscles generally) (but brain dies if it doesn't get fresh blood supply within 15 minutes as it can utilize only glucose)
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Without adequate blood supply the human leg can only survive six hours without blood flow. If blood flow is not re established within those six hours amputation may become necessary.
They can probably last 2 or 3 seconds.
actually the heart continues to beat for several minutes