Microhematocrit tubes are read according to PCV (Packed Cell Volume).
After centrifuging the tube, you will need a PCV card reader to determine the amount of packed red blood cells within the sample of blood. The PCV is determined by aligning the top of the clay plug (just as it reaches the layer of red blood cells) on the zero line and then gently "rolling" the tube up the chart until the intersecting middle line is aligned where the packed red cells and Buffy coat meet. Read across the chart and you should have a percentage of red blood cells to record as a PCV.
Plug one end with clay
A standard microhematocrit tube with a capacity of about 75-80 microliters is typically used for performing a white blood cell count.
Microhematocrit tubes are read according to PCV (Packed Cell Volume). After centrifuging the tube, you will need a PCV card reader to determine the amount of packed red blood cells within the sample of blood. The PCV is determined by aligning the top of the clay plug (just as it reaches the layer of red blood cells) on the zero line and then gently "rolling" the tube up the chart until the intersecting middle line is aligned where the packed red cells and Buffy coat meet. Read across the chart and you should have a percentage of red blood cells to record as a PCV.
Spun microhematocrit tubes are used to measure hematocrit levels, which represents the proportion of red blood cells to the total volume of blood. This test is helpful in diagnosing conditions such as anemia or dehydration, as well as monitoring an individual's response to treatment.
For a microhematocrit test, capillary blood, usually obtained from a finger or heel stick, is used. The blood sample is placed into a special capillary tube and spun in a centrifuge to separate the components, allowing for measurement of the packed cell volume (hematocrit).
The speed and time of the centrifuge directly affect the microhematocrit values. Errors can be caused if it is not spun at the correct speed.
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capillary blood
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