Nope. "Germs" for use in laboratories are often stored at temperatures far below anything in nature or a normal refrigerator. Once they warm up to their preferred temperature, they function normally. As always, there are exceptions to this, but likely not many.
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Yes it does. Its also a reason why stores in the winter keep the store cold to keep germs from traveling
Yes it can because when it gets cold all the germs like disappear and yep it kills germs