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Usually, the vaccum in the tube is such that it cannot really be overfilled. Sometimes, blood is collected using a plain syringe and then put into a blue top. This can easily be the cause of cause overfilling. As for the effects, the ratio of the blood to anticoagulant would be such that there is less anticoagulant. This can cause the specimen to clot, and/or slightlydecrease the coagulation results. Less anticoagulant, clots faster.

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Q: Can a citrate tube be overfilled with blood If so what are the effects?
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