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What kind of molecule must be labeled to make radioactively labeled DNA?
Radioactive nucleotide
What enzyme us used to make radioactively labeled DNA?
DNA polymerase
What kind of molecule must be and ldquolabeled and to make radioactively labeled DNA?
To create radioactively labeled DNA, the molecule that must be labeled is a nucleotide. Specifically, one of the nucleotides (adenine, thymine, cytosine, or guanine) can be modified to include a radioactive isotope, such as phosphorus-32 or tritium. When these labeled nucleotides are incorporated into a DNA strand during replication or synthesis, the entire DNA molecule becomes radioactively labeled. This technique is often used in molecular biology for various applications, including tracking DNA synthesis and conducting hybridization experiments.
Can be used to compare the amount of radioactively labeled DNA in two or more samples?
Scintillation counter
Can be use to determine the precise amount of radioactively labeled DNA in a chemical solution?
scintillation counter. APEX
Can DNA polymerase be used to determine the general location of radioactively labeled DNA?
Geiger Counter
You briefly expose bacteria undergoing DNA replication to radioactively labeled nucleotides when you centrifuge the DNA isolated from the bacteria the DNA separates into two classes one class of la?
The DNA separated into two classes: labeled DNA and unlabeled DNA. The labeled DNA contains the radioactively labeled nucleotides that were incorporated during DNA replication, while the unlabeled DNA represents the original, non-radioactively labeled DNA from the bacteria. The centrifugation process separated the DNA based on density, with the heavier labeled DNA migrating to a higher position in the centrifuge tube compared to the unlabeled DNA.
You label cells with a radioactive isotope of sulfur (35S). The only molecules that would be radioactively labeled in these cells are?
proteins and RNA, as sulfur is commonly found in cysteine and methionine amino acids, which are building blocks of proteins. Additionally, sulfur is present in RNA molecules in the form of thiol groups and nucleotides like thymine.
Using radioactively labeled DNA probes in studies of heredity makes it possible for scientist to do what?
Study the inheritance of traits that are not seen as a phenotype
What are radioactively tagged bacteriophages to discover that DNA not protein is injected into host cells?
Radioactively tagged bacteriophages are used to confirm that DNA, not protein, is injected into host cells during infection. The radioactively labeled DNA can be detected inside the host cells after infection, providing evidence that DNA is the genetic material transferred by the bacteriophages. This experiment was crucial in establishing DNA as the genetic material in organisms.
In the Hershey and Chase experiment that helped confirm that DNA not protein was the hereditary material the key finding was that?
the key finding was that DNA, labeled with radioactive phosphorus, was the material that entered the bacterial cells during viral infection and was ultimately responsible for the transmission of genetic information, rather than the proteins also present in the viral coat.
What of the following reactions is used to radioactively label DNA?
The reaction that is commonly used to radioactively label DNA is the nick translation method, where a DNA molecule is treated with a DNA polymerase, dNTPs (including radioactive ones), and a DNAase to create radioactive labeled DNA fragments.
