Eeyore is neither, he's a donkey ;)
But he is a boy donkey, despite Disney illustrating him with a pink bow in his tail. A A Milne, the author of the Winnie the pooh stories, describes Eeyore as 'he' and 'him' throughout the books. Here are a couple of examples, with references to Eeyore emboldened for clarity:
"And anyhow, Eeyore was in the river, and now hewasn't, so he hadn't done any harm.
'How did you fall in, Eeyore?' asked Rabbit, as he dried him with Piglet's handkerchief."
(from Pooh Invents A New Game)
"... Pooh and Piglet were sitting in the Thoughtful Spot and wondering.
'What I think,' said Pooh, 'is I think we'll go to Pooh Corner and see Eeyore, because perhaps his house has been blown down...'"
(from Piglet Does A Very Grand Thing)
and:
"One day when Pooh was thinking, he thought he would go and see Eeyore, because he hadn't seen him since yesterday."
(from Tiggers Don't Climb Trees)
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Winnie the pooh i feel was male.
Winnie the Pooh is a boy not a girl, it clearly states in the storybooks Winnie the Pooh that it says HE for Winnie the Pooh. ""Well," said Pooh, "what I like best -- " and then he had to stop and think. Because although Eating Honey was a very good thing to do" AA Milne
Owl is a male.
Winnie the Pooh is a boy.
Winnie the Pooh is a boy. Despite his cute little red shirt and affinity for honey, he's definitely a he. But hey, if he wants to rock that crop top and be in touch with his feminine side, more power to him!