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Because some things that you might look at under a microscope are transparent and hard to see. Adding Methylene Blue to the slide would dye the stuff blue.....i think.

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Q: Why did you need to add methylene blue to the slide?
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What is methylene blue use for when gram staining?

Methylene blue can be used to prepare slide for animal cells. For example, if you want to examine a piece of your cheek cell, you would take a piece of cotton wool and rub it on the inside of your cheek and the rub it on a microscope slide, the you add a little distilled water and then a drop of methylene blue. The final step you would take, is to cover the slide with a cover slip, then place it under the microscope.


Why is methylene blue necessary to view your check cells?

This is because the cheek cell is transparent. Since the membrane of the cheek cell is selectively permeable, it allows the methylene blue to enter the cell , therefore makes it blue in color from the inside. Then we are able to see the cheek cell properly under a microscope... please note. : we are supposed to add a bit of water and gliserene too . WATER : to transfer the cheek cells on to the slide AND GLISERENE: to prevent the cheek cells from drying up.


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