Defeat makai is a quest item that is needed to unlock certain rooms in Tercesssuinotlim. You need 50 Defeated Makai. This can be gained by killing Dark Makai, also found in tercesssuinotlim.
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You can go to Yokai Ferry (/join yokaiboat) and start the next part of the storyline in Akiba - the next Chaos Lord.
Kuro is the boss in the river in aqworlds.
Go to US.CODEJUNKIES.COM or UK.CODEJUNKIES.COM
You can't exactly be a Guardian on AQWorlds, but you can verify on AQW that you have Guardian on the original Adventure Quest and get Guardian class in AQWorlds. :)
You can find Dark Makai in the area "Miltonius' Secret" which can be accessed through the citadel area. You will need 50 bone dust which can be obtained by defeating any skeletal monster to proceed.