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No.
Originally, the developers at Nintendo had intended for the two to be related, but eventually decided against it. This can easily be seen by common physical traits between the two and between Peach and Rosalina's mother, as seen within the storybook of Super Mario Galaxy.
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Earn +20 ptsQ: Are Princess Peach and Princess Rosalina sisters?
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Who is prettier Princess Peach or Rosalina?
princess peach
Are peach daisy and rosalina sisters?
No, Rosalina and peach are sisters. Daisy is peaches cousin.
Are Peach and Rosalina sisters?
yes they are sisters
Who is princess peach's sister?
Princess peach does not have a sister, Princess Daisy is in fact another princess from Sarasaraland. In early concepts Princess Rosalina was meant to be Princess's Peach's sister but the idea was scrapped
Who is princess Rosalina married to in Mario?
No one. But she likes Mario. However, she does not like Mario's girlfriend, Princess Peach.
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