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Usually csf samples are collected in 4 bottles. Bottle1, 0.5ml with flouride for sugar estimation. Bottle2, 1ml for microbiology studies. Bottle3, 0.5ml for hematology (cell count) and protein. Bottle4 for other special studies like immunological studies, electrophoresis, etc. Here we should never refrigerated the bottle2 if it is for bacterial studies. Bcz Haemophilus influenzae and Neisseria meningitidis may not survive the cold temperature. In case of delay sample should be kept at 37 degree Celsius. But viral samples can be refrigerated at 2-6 degree Celsius.

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