No. Although unicorns sometimes appear to be mentioned in the Bible, they are actually mythical creatures. We also know from science that there was never a worldwide Flood that required the legendary Noah to build an Ark - although it is suggested that the story is based on folk memories of the inundation of what is now the Black Sea.
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No. As the song says:
'And Noah looked out through the driving rain.
Those unicorns were hiding, playing silly games.
A kicking and a splashing while the rains were pouring.
All those silly unicorns.'
Though the Flood was a historical event recorded by societies in every region of the globe, the existence of unicorns is not proven. Many other creatures, including dinosaurs, were not on Noah's boat since that (their demise) was part of God's plan.
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Unicorns have never existed, they are mythological creatures. The flood story and Noah's Ark are also works of fiction. Nevertheless, Unicorns are not mentioned in the flood story.